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Preclinical validation includes rabbit HCC models demonstrating significant histological advantages of IRE over RE and untreated controls: IRE zones exhibited 51-60% fewer tumor cells, 66-67% reduced microvasculature, and 185-228% increased cell death ( in vitro with distinct ultrastructural changes observed via TEM, achieves complete ablation in rabbit VX2 liver tumors under contrast-enhanced ultrasound guidance ( + extracellular vesicle release, causing CD8 + T-cell dysfunctionan effect reversed by PD-L1 blockade to significantly suppress tumor growth and improve survival ( + T-cell and dendritic cell infiltration in peri-ablational zones ( + T cell necroptosis yet attenuating pro-tumor inflammatory populations ( + mononuclear phagocyte differentiation and memory CD8 + T-cell formation in human trials ( 3.3 Prostate cancer Emerging clinical evidence establishes IRE as a safe and effective modality for prostate cancer treatment, with recent studies highlighting its capacity to potentiate checkpoint immunotherapy